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Basics of Extracellular Vesicles
Course
en
English
10 h
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- Self-paced
- Free Access
- Fee-based Certificate
- 5 Sequences
- Intermediate Level
Course details
Syllabus
- Week 1 - Introduction to the Course and the Field of Extracellular Vesicles
In this module, we will introduce the course and the field of extracellular vesicles. We will discuss the nomenclature and the history of extracellular vesicles as well as the organisms and places that have been shown to release/contain extracellular vesicles.... - Week 2 - Biogenesis, Cargo and Uptake of Extracellular Vesicles
In this module, we will focus on the biogenesis and release of extracellular vesicles as well as the uptake mechanisms of extracellular vesicles when they are encountered by a recipient cell. This module also covers the different RNA, protein and lipid types p... - Week 3 - Collection and Processing of Cell Culture Media and Body Fluids Prior to Isolation of Extracellular Vesicles
In this module, we will focus on the collection and processing of cell culture media and body fluids prior to isolation of extracellular vesicles. We will discuss some of the considerations and guidelines that are important during the collection of staring mat... - Week 4 - Methods for Isolating Extracellular Vesicles
This module highlights some of the different methods used to isolate extracellular vesicles. Here the basic concepts for methods such as differential ultracentrifugation, density gradient, size exclusion and kit-based precipitation will be covered and some gui... - Week 5 - Techniques for Characterization and Quantification of Extracellular Vesicles
This module highlights some of the different techniques used to characterize extracellular vesicles. Here the basic concepts for techniques such as electron microscopy (EM), cryo-TEM, flow cytometry, atomic-force microscopy and nanoparticle tracking analysis w...
Prerequisite
None.
Instructors
Cecilia Lasser
Researcher
Krefting Research Centre, University of Gothenburg
Editor
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